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. 2012 Mar 14;7(3):e33245. doi: 10.1371/journal.pone.0033245

Figure 9. APCP-induced oxidative burst elicited short-term adaptive responses in mammalian cells.

Figure 9

Human keratocytes were exposed for 2 minutes to APCP and cultured under optimal conditions. The mRNA transcript levels specific for human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2, panel A), DNA glycosylase OGG1 (panel B) and glutathione peroxidase 1 (GPX1, panel C) were evaluated by quantitative real-time PCR. Expression of the target genes was normalized to the endogenous levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are expressed as mean ± SE (error bars) of gene copies in 5 µg of loaded total RNA, obtained from two independent experiments. * P<0.05 vs untreated cells. Protein expression of GPX and OGG1 in keratocytes was confirmed by Western blot analysis of samples taken at different time points after APCP treatment (panel D).