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. 2012 Mar 14;7(3):e33245. doi: 10.1371/journal.pone.0033245

Table 1. Genes, related primer sequences, UPL probes and annealing temperatures relative to amplicons evaluated by quantitative RT-PCR.

Gene (accession n) Primer Sequences UPL probe T (°C)
GADPH (NM_002046) fw: 5′-cgggaagcccatcacca-3′ rv: 5′-ccggcctcaccccatt-3′ 60 60
NFE2L2 (NM_006164.3) fw: 5′-acacggtccacagctcatc-3′ rv: 5′-tgcctccaaagtatgtcaatca-3′ 3 60
OGG1 (NM_016827.2) fw: 5′-cttttggacctcggctcat-3′ rv: 5′-caaatgcattgccaaggag-3′ 20 60
GPX1 (NM_000581.2) fw: 5′-caaccagtttgggcatcag-3′ rv: 5′-gttcacctcgcacttctcg-3′ 77 60

fw: forward; rv: reverse; Ta: annealing temperature.