Table 2. APCP-induced ROS formation in bacteria.

	E. coli	S. aureus	P. aeruginosa
Untreated	3.81±1.46	0.41±0.08	1.52±0.31
30 sec after APCP	10.96±0.62*	32.60±1.39*	12.89±1.23*
60 sec after APCP	29.25±5.84* §	32.73±0.93*	16.94±0.34*
120 sec after APCP	33.64±6.55* §	35.25±0.53*	19.48±0.74* §

Intracellular ROS generation was measured using flow cytometry in bacteria loaded with H₂DCFDA. Bacteria were treated with APCP for 2 minutes and analyzed at 30, 60 and 120 seconds after treatment. Data are expressed as the mean ± SE (percentage of fluorescence) of at least three independent experiments.

^* p<0.05 vs untreated bacteria;

^§ p<0.02 vs samples collected 30 seconds after APCP treatment.