Table 2. Molecular phosphatidylcholine (PC) species and their distribution in shrimp ovaries.
m/z | Molecular species | adduct | Distribution |
756.5 | 16∶0/16∶1 | Na | Oocyte stages 1–2 |
780.5 | 16∶0/18∶2 | Na | Oocyte stages 3–4 |
782.5 | 16∶0/18∶1 | Na | Oocyte stages 3–4 |
802.5 | 16∶1/20∶4 | Na | All stages |
804.5 | 18∶2/18∶2 | Na | Oocyte stages 3–4 |
806.5 | 18∶1/18∶2 | Na | Oocyte stages 3–4 |
808.5 | 18∶0/18∶2 | Na | Oocyte stages 3–4 |
820.5 | 16∶0/20∶4 | K | Oocyte stages 3–4 |
826.5 | 18∶2/20∶5 | Na | Oocyte stages 3–4 |
828.5 | 16∶0/22∶6 | Na | All stages |
830.5 | 18∶1/20∶4 | Na | Oocyte stages 3–4 |
832.5 | 18∶0/20∶4 | Na | Oocyte stages 3–4 |
844.5 | 16∶0/22∶6 | Na | Oocyte stages 3–4 |
850.5 | 18∶3/22∶6 | K | All stages |
852.5 | 18∶2/22∶6 | Na | Oocyte stages 3–4 |
854.5 | 18∶1/22∶6 | Na | All stages |
870.5 | 18∶1/22∶6 | K | Oocyte stages 3–4 |
874.5 | 20∶5/22∶6 | Na | All stages |
900.5 | 22∶6/22∶6 | Na | All stages |
All PC species were verified by MS/MS analysis; however, complete fragment ion information was difficult to obtain for some PC molecules. Therefore, lipid molecules for which only partial fragment ions could be obtained were identified by an online database search (http://www.hmdb.ca/labm/jsp/mlims/MSDbParent.jsp) and Simlipid software version 1.0. The underlined molecules were identified from MS/MS analysis and had complete fragment ion spectra.