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. 2012 Mar 14;7(3):e33360. doi: 10.1371/journal.pone.0033360

Figure 5. Effect of MSCs on cytokine production in the inflamed colon.

Figure 5

Colonic explants were cultured for 24 h at 37°C. After centrifugation, supernatants were used for measurement of the concentration of cytokines by an ELISA method for rat TNF-α (A), IL-1β (B), VEGF (C), TGF-β (D), and IL-10 (E), as described in Supplementary Materials. AT-, BM-MSC, and vehicle were administered to colitic animals, while control group did not receive any treatment. Values are expressed as picogram of cytokine/mL of culture supernatant, and normalized by protein contents. Horizontal bars represent medians, boxes represent the 25th and 75th percentiles, and vertical bars represent ranges of 10 animals/group. Differences were evaluated with the one-way ANOVA on ranks with Dunnett's test.