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. 2012 Mar 14;7(3):e33372. doi: 10.1371/journal.pone.0033372

Figure 1. Protein characterization.

Figure 1

(A) SEC chromatogram of 1 g/L light chain solution in 25 mM PBS buffer at pH 7.4 (―) and in 20 mM HCl at pH 2.0 (–). Insertion: calibration curve obtained with bovine serum albumin (60 kDa), chymotrypsinogen A (25 kDa) and lysozyme (14.5 kDa); (B) and (C) Mass spectroscopy analysis in 25 mM PBS buffer at pH 7.4 (B) and in 20 mM HCl at pH 2.0 (C).