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. 2012 Mar 14;7(3):e33712. doi: 10.1371/journal.pone.0033712

Figure 4. Effects of CoQ10 supplementation on oxidative stress induced by Rotenone and t-Butyl hydroperoxide (TBH).

Figure 4

ROS were detected following DCFDA fluorescence in cells treated for 24 hours with 100 nM or 10 uM native CoQ10 or Qter®. ROS were induced by 48 hours treatment with 100 nM Rotenone in T67 (A) and H9c2 cells (B) or by 30 minutes exposure to 100 µM TBH in T67 cells (C) and H9c2 cells (D). Data are the mean ± S.D. of at least three different determinations and are expressed as arbitrary fluorescence units (A.F.U.) normalized on protein content. Protein content was evaluated by Lowry method. Asterisks refer to the statistically significant decrease of ROS production in Rotenone/TBH treated samples supplemented with quinones (n = 5, * p≤0.05); ** p≤0.001).