Figure 9. Assessment of mitochondrial potential by JC-1 staining.
Representative images show JC-1 fluorescence in control H9c2 cells (A), H9c2 cells treated for 24 hours with 100 nM Qter® (B) and H9c2 cells treated with 500 nM of the uncoupler FCCP (C). Panel D shows quantitative analysis of Red/Green fluorescence ratio measured by ImageJ software. (n = 20, * p≤0.001 vs. control; ≠p≤0.001 vs. control.).