Figure 4.

Stereo pair of endothelial cells in the renal artery double-stained with rhodamine-labeled phalloidin (a) and antiphosphotyrosine antibody (PY-20) (b). A merged image (a and b) is shown in (c). The three-dimensional distributions of actin filaments (c: red) and tyrosine-phosphorylated proteins (c: green) are shown. The region of overlap between actin filaments and tyrosine-phosphorylated protein is shown in yellow (c). Tyrosine-phosphorylated proteins were observed below the plasma membrane, mainly at sites of cell-to-cell apposition and on the apical surface of the endothelial cells. To see the stereo image, use special glasses or a light source split into the viewer's eyes. The focal plane closer to the viewer is the apical surface of the renal endothelial cells. The arrow indicates the direction of blood flow. Bar: 50 μm.