Table 2.
Primers used for real-time RT-PCR analysis.
| Genes | Primer name | Primer sequences 5′→3′ |
|---|---|---|
| Sry | Sry-F | AGCTCTTACACTTTAAGTTTTGAC |
| Sry-R | GCAGCTCTACTCCAGTCTTGCC | |
| Wt1(−KTS) | Wt1(−KTS)-F | TACCTTAAAGGGCCACGGTAT |
| Wt1(−KTS)-S | GAAGGGCTTTTCACCTGTATGA | |
| Wt1(+KTS) | Wt1(+KTS)-F | GCTGGGAGCTCCAGCTCAGT |
| Wt1(+KTS)-R | GGGCTTTTCACTTGTTTTAC | |
| Sox9 | Sox9-F | GGTGCTGAAGGGCTACGCTGGACG |
| Sox9-R | GGCTGGTACTTGTAATCGGGTGGTC | |
| Gata4 | Gata4-F | CGAGGGTGAGCCTGTATGTAA |
| Gata4-R | TGGCCTGCGATGTCTGAGTG | |
| Amh | Amh-F | TCCTACATCTGGCTGAAGTGATATGGGAGC |
| Amh-R | CTCAGGGTGGCACCTTCTCTGCTTGGTTGA | |
| Sf1 | Sf1-F | TGGTGTCCAGTGTCCACCCTTAT |
| Sf1-R | TCCGTCACGTGTAATGCTTGT | |
| Dax1 | Dax1-F | AGAGTATGCCTATCTGAAAGGGACCGTG |
| Dax1-R | ACGACATCGCTATTGATGAATCTCAGCA | |
| β-actin | β-actin-F | AACCCTAAGGCCAACCGTG |
| β-actin-R | CAGGATTCCATACCCAAGAAGG |