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(a) Western blot assay of TLR4 following Ad-siTLR4 or Ad-EGFP pretreatment in LPS treated AMs. (b) Real-time PCR analysis of TLR4 mRNA expression in LPS-treated AMs. (c) and (d) levels of TNF-α and IL-1β in the supernatant. *P < 0.05 versus Ad-EGFP group.
