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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Steroids. 2012 Jan 20;77(5):477–483. doi: 10.1016/j.steroids.2012.01.007

Figure 1.

Figure 1

H292 cells express CYP24 in vitro and in vivo. (A) H292 cells were treated with 1 nM 1,25(OH)2D3. At the times indicated, cells were harvested and RNA was prepared. CYP24 mRNA expression was determined by QPCR, as detailed in methods. Results are the average of two separate determinations. (B) H292 cells were exposed to 100 nM 1,25(OH)2D3 in the absence (white bars) or presence (black bars) of CTA091 (50 nM). At various times, tissue culture homogenates were prepared and 1,25(OH)2D3 concentrations determined by LC-MS/MS. Values represent the mean +/− SD for triplicate determinations. (C) Mice bearing H292 xenografts were treated with a single dose of 1,25(OH)2D3. At various times, tumors were harvested for determination of CYP24 mRNA levels by QPCR.