Effect of pharmacological blockade of MR and GR on dexamethasone-induced Edn1 gene expression in mIMCD-3 cells. Pharmacological inhibition of MR or GR on dexamethasone-stimulated Edn1 mRNA was evaluated in mIMCD-3 cells treated with vehicle (veh, open bars), 1 μM dexamethasone (dex, closed bars), or 1 μM dexamethasone in the presence of 10 μM spironolactone (spiro) or 10 μM RU486 for 1 h. Edn1, and Sgk1 mRNA levels were determined by QPCR, normalized to actb (β-actin) and expressed as mRNA fold change relative to vehicle ± SE. (*p < 0.05, **p < 0.001 relative to vehicle; n ≥ 3)