Table 1.
Summary of the GC-NCI-MS method validation
| TFAA-DNPH (ng injected) | Repeatability
|
|||
|---|---|---|---|---|
| Area Ratio (RSD %)a |
Retention Time (RSD %)b |
|||
| Intra-dayc | Inter-dayd | Intra-dayc | Inter-dayd | |
| 5 | 1.1 | 5.5 | 0.02 | 0.02 |
| 10 | 6.5 | 7.1 | 0.01 | 0.01 |
| 100 | 2.1 | 3.2 | 0.02 | 0.80 |
| CQe (ng/μL) | Accuracy
|
||
|---|---|---|---|
| CMf ± SD (ng/μL) | Precision (RSD %) | Accuracy (%) | |
| 5 | 5.58 ± 0.03 | 0.5 | 11.6 |
| 50 | 50.6 ± 1.6 | 3.2 | 1.2 |
| 150 | 147 ± 3 | 2.1 | −2.1 |
a
Area Ratio (RSD %) denotes precision expressed in RSD % of analyte/IS area ratios.
b
Retention Time (RSD %) denotes precision expressed in RSD % of retention times.
c
n = 5.
d
n = 15.
e
CQ denotes the nominal concentration of TFAA-DNPH in QC samples.
f
CM denotes the measured concentration of TFAA-DNPH in QC samples.