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Michaelis-Menten kinetic parameters for hVACVase-mediated hydrolysis (mean ± SEM; n=3).
| Km (μM) | Vmax (nmol/min/μg) | kcat (s−1) | kcat/Km (mM−1·s−1) | |
|---|---|---|---|---|
| Val-3-HPG | 46 ± 5 | 321 ± 9 | 154 ± 4 | 3370 ± 460 |
| Ile-3-HPG | 9.0 ± 1.2 | 30 ± 1 | 14 ± 1 | 1580 ± 260 |
| Phe-3-HPG | 207 ± 16 | 718 ± 16 | 345 ± 8 | 1660 ± 160 |
| valacyclovir | 68 ± 4 | 120 ± 2 | 58 ± 1 | 850 ± 66 |
