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Primer sequences for Semi-quantitative PCR
| mRNA or Gene (Accession ID) | Oligonucleotide sequence (5'-3') | Anneling Tm(°C) | Product Length (bp) |
|---|---|---|---|
| P53 | F- GTGGAAGGAAATTTGCGTGTG | 59 °C | 180 |
| NM_001126117.1 | R- CCAGTGTGATGATGGTGAGGATG | ||
| Bcl-2 | F- TGCACCTGACGCCCTTCAC | 59 °C | 291 |
| NM_000633.2 | R- AGACAGCCAGGAGAAATCAAACAG | ||
| β-actin | F- TTCTACAATGAGCTGCGTGTGG | 59 °C | 119 |
| NM_001101.3 | R- TTCTACAATGAGCTGCGTGTGG |
