Table 2.
Primers designed according to am1 gene sequence.
| Primer | Sequence 5’ to 3’ | Amplification region | Tm °C |
|---|---|---|---|
| F1 | CCTCCTTCAACCGTTACCCCG | Arg 10 –Pro12 site | 75 |
| R1 | GCGAGAAGGATACCAAGGCAG | Conserved sequence | 73.2 |
| F2 | CTTCTCGCCAGTGGTAGGGTCA | Conserved sequence | 75 |
| R2 | GAAGATACTCCCATCGACCCCT | Conserved sequence | 73.2 |
| F3 | GCTAACGAACTTGCGAGGTAGA | FDP ionization site | 71.3 |
| R3 | CGTTTCCTCCCTTCTTGTCTAG | Conserved sequence | 71.3 |
| F4 | CGGACTTGGATCAGGGGTTTTC | Active site | 73.2 |
| R4 | ATGGTTAGGAAGCACGTATCGG | Catalytic site | 71.3 |
| F5 | GCTTAAAGGGAAACGGCAAC | Start point | 68.9 |
| R5 | CATGATGTGTATAGCGTGC | Catalytic site | 65.4 |