Table 3. Affinity of other amino acid methyl esters for the four cluster II CR domains.

n.d., not determined.

	Histidine methyl ester		Aspartate methyl ester		Alanine methyl ester
	Kd (mM)	ΔG° (kJ/mol)	Kd (mM)	ΔG° (kJ/mol)	Kd (mM)	ΔG° (kJ/mol)
CR3
pH 5.5	1.2±0.07	−16.7	83±17	−6.2	n.d.
pH 7.4	n.d.		66±11	−6.7	n.d.
CR5
pH 5.5	0.33±0.01	−19.9	59±2	−7.0	4.1±0.24	−13.6
pH 7.4	n.d.		63±5	−6.8	n.d.
CR8
pH5.5	0.29±0.01	−20.2	78±3	−6.3	4.9±0.25	−13.2
pH 7.4	n.d.		119±17	−5.3	n.d.
D1085A
pH 5.5	4.6±0.05	−13.3	235±15	−3.6	n.d.
pH 7.4	n.d.		263±19	−3.3	n.d.