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. Author manuscript; available in PMC: 2012 Oct 3.
Published in final edited form as: FEBS Lett. 2011 Aug 28;585(19):3058–3064. doi: 10.1016/j.febslet.2011.08.028

Fig. 3.

Fig. 3

RIP1-unrelated protective effects of Nec-1 against NO donor-induced β-cell death. (A) GSNO-induced cell death was significantly prevented by Nec-1 (N), a potent inhibitor of RIP1, and its analogue, Nec-1i (I), which is incapable of inhibiting RIP1, in βTC-6 and INS-1/832 cells. *p<0.01, **p<0.0001 vs without GSNO, #p<0.001 vs GSNO (100 or 300 μM) without Nec-1, §p<0.001 vs GSNO (200 or 500 μM) without Nec-1, †p<0.01 vs GSNO 300 μM+Nec-1, p<0.001 vs GSNO (200 or 500 μM)+Nec-1. (B) siRNA-mediated knockdown of RIP1 (siRIP1) did not block GSNO (100 μM)-induced cell death in βTC-6 cells, as compared with control siRNA. Moreover, Nec-1 (100 μM) effectively inhibited GSNO (100 μM)-induced cell death in βTC-6 cells transfected with siRNA for RIP1 as well as control siRNA. *p<0.05, **p<0.0001 vs without GSNO, #p<0.05 vs GSNO+control siRNA without Nec-1, §p<0.0001 vs GSNO+control siRNA+Nec-1. (C, D) Nec-1 and Nec-1i almost completely reversed decreased Akt phosphorylation in GSNO-treated INS-1/832 cells. IB: immunoblotting.