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. Author manuscript; available in PMC: 2012 Oct 3.
Published in final edited form as: FEBS Lett. 2011 Aug 28;585(19):3058–3064. doi: 10.1016/j.febslet.2011.08.028

Fig. 4.

Fig. 4

Effects of NO donor and Nec-1 in wild-type and RIP1-deficient Jurkat cells. (A) GSNO induced cell death in control wild-type (RIP1-proficient) Jurkat cells in a dose-dependent manner. Both Nec-1 (N) (100 μM) and Nec-1i (I) (100 μM) inhibited GSNO-induced cell death in wild-type Jurkat cells. **p<0.0001 vs without GSNO, ##p<0.0001 vs GSNO 400 μM without Nec-1, §§p<0.0001 vs GSNO 600 μM without Nec-1. (B) GSNO (600 μM) induced cell death in both wild-type (RIP1+ or WT) and RIP1-deficient (RIP1−) Jurkat cells. Nec-1 (100 μM) significantly decreased GSNO-induced cell death in both wild-type and RIP1-deficient Jurkat cells. Immunoblotting confirmed that RIP1 was expressed in wild-type, but not RIP1-deficient, Jurkat cells. *p<0.001, **p<0.0001 vs without GSNO, #p<0.001 vs WT with GSNO alone, §p<0.0001 vs GSNO+Nec-1. Cell death was assessed by Trypan blue staining. IB: immunoblotting.