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. 2012 Mar 13;78(11):833–835. doi: 10.1212/WNL.0b013e318249f776

Figure. Denervation precedes inflammation in peripheral nerves of amyotrophic lateral sclerosis (ALS) mice.

Figure

(A, B) Quantitative RT-PCR (qRT-PCR) demonstrated the reappearance of the acetylcholine receptor (AChR) fetal γ subunit mRNA in diaphragm and gastrocnemius muscles occurs at 55 days of age in ALS mice. (C, D) Increased expression of CD68 mRNA was first noted at 77 days of age in phrenic and sciatic nerves of ALS mice. (E) Immunohistochemistry for CD68 corroborated the qRT-PCR result that at 77 days of age inflammation was increased in phrenic nerve of ALS mice. (F) Immunohistochemistry and qRT-PCR for CD68 demonstrates that inflammation was evident earlier in the lumbar spinal cord compared with the cervical spinal cord of ALS mice. ap < 0.05 vs wild-type (WT), bp < 0.01 vs WT, cp < 0.01 vs 10-day-old WT mice, dp < 0.05 vs 10-day-old mSOD1 mice, ep < 0.01 vs 10-day-old mSOD1 mice, fp < 0.05 vs 20-day-old mSOD1 mice, and gp < 0.05 vs 55-day-old mSOD1 mice. In panel E, scale bars = 50 μm. In panel F, scale bars = 200 μm.