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. 2012 Mar 9;7(3):e31253. doi: 10.1371/journal.pone.0031253

Table 1. Percentages of the different species observed by SEC.

[protein] (µM) M (%) D (%) O (%)
BlaP 50 95.1 4.9 0
120 94.4 5.6 0
BlaP(Gln)23 50 99.4 0.6 0
120 99.3 0.7 0
BlaP(Gln)30 50 99.6 0.4 0
120 99.5 0.5 0
BlaP(Gln)55 50 98.9 1.1 0
120 98.6 1.4 0
BlaP(Gln)79 10 97.3 0.9 1.8
50 96.4 1.1 2.5
120 95.3 1.2 3.5

This analysis was carried out using a Superdex 200 GL 10/300 column using PBS buffer, pH 7.5. M, D, O stand for monomeric, dimeric, and high molecular weight oligomeric species, respectively.