Table 1. Percentages of the different species observed by SEC.
[protein] (µM) | M (%) | D (%) | O (%) | |
BlaP | 50 | 95.1 | 4.9 | 0 |
120 | 94.4 | 5.6 | 0 | |
BlaP(Gln)23 | 50 | 99.4 | 0.6 | 0 |
120 | 99.3 | 0.7 | 0 | |
BlaP(Gln)30 | 50 | 99.6 | 0.4 | 0 |
120 | 99.5 | 0.5 | 0 | |
BlaP(Gln)55 | 50 | 98.9 | 1.1 | 0 |
120 | 98.6 | 1.4 | 0 | |
BlaP(Gln)79 | 10 | 97.3 | 0.9 | 1.8 |
50 | 96.4 | 1.1 | 2.5 | |
120 | 95.3 | 1.2 | 3.5 |
This analysis was carried out using a Superdex 200 GL 10/300 column using PBS buffer, pH 7.5. M, D, O stand for monomeric, dimeric, and high molecular weight oligomeric species, respectively.