Table 3. Thermodynamic parameter values of unfolding of wild-type and chimeric BlaP.

	ΔG°NU(H2O) (kJ·mol−1)	−m NU (kJ·mol−1·M−1)	Cm (M)	Tm app (°C)
BlaP	37.0±1.8	13.5±0.6	2.74±0.23	58.5±0.4
BlaP(Gln)23	29.3±2.4	13.7±0.9	2.13±0.19	53.9±0.7
BlaP(Gln)30	29.4±1.2	15.9±0.7	1.85±0.15	53.7±0.3
BlaP(Gln)55	29.0±0.6	15.6±0.6	1.86±0.15	53.5±0.6
BlaP(Gln)79	28.2±1.3	14.9±0.6	1.89±0.17	53.2±0.3

The values were deduced from the analysis of unfolding transitions monitored by intrinsic fluorescence and far-UV CD measurements. The urea unfolding experiments were carried out at 25°C. ΔG°_NU(H₂O), m _NU, C_m and T_m ^app values result from the mean of the values obtained by far-UV CD and intrinsic fluorescence; note that for thermal unfolding, three measurements were carried out both by fluorescence and CD. Thermal unfolding was not fully reversible and only the apparent mid-unfolding temperature (T_m ^app) was determined.