Abstract
Specific in vitro transcription by partially purified RNA polymerase from Methanobacterium thermoautotrophicum of DNA sequences cloned in between the promoter and terminator regions of the methyl CoM reductase transcription unit of the same organism is described. The 5'-terminus of the product has been mapped. Deletion analyses of the promoter region show the limits of the sequences essential for the promoter function.
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- Allmansberger R., Knaub S., Klein A. Conserved elements in the transcription initiation regions preceding highly expressed structural genes of methanogenic archaebacteria. Nucleic Acids Res. 1988 Aug 11;16(15):7419–7436. doi: 10.1093/nar/16.15.7419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berghöfer B., Kröckel L., Körtner C., Truss M., Schallenberg J., Klein A. Relatedness of archaebacterial RNA polymerase core subunits to their eubacterial and eukaryotic equivalents. Nucleic Acids Res. 1988 Aug 25;16(16):8113–8128. doi: 10.1093/nar/16.16.8113. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bokranz M., Bäumner G., Allmansberger R., Ankel-Fuchs D., Klein A. Cloning and characterization of the methyl coenzyme M reductase genes from Methanobacterium thermoautotrophicum. J Bacteriol. 1988 Feb;170(2):568–577. doi: 10.1128/jb.170.2.568-577.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown J. W., Daniels C. J., Reeve J. N. Gene structure, organization, and expression in archaebacteria. Crit Rev Microbiol. 1989;16(4):287–338. doi: 10.3109/10408418909105479. [DOI] [PubMed] [Google Scholar]
- Brown J. W., Thomm M., Beckler G. S., Frey G., Stetter K. O., Reeve J. N. An archaebacterial RNA polymerase binding site and transcription initiation of the hisA gene in Methanococcus vannielii. Nucleic Acids Res. 1988 Jan 11;16(1):135–150. doi: 10.1093/nar/16.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guo L. H., Wu R. Exonuclease III: use for DNA sequence analysis and in specific deletions of nucleotides. Methods Enzymol. 1983;100:60–96. doi: 10.1016/0076-6879(83)00046-4. [DOI] [PubMed] [Google Scholar]
- Huet J., Schnabel R., Sentenac A., Zillig W. Archaebacteria and eukaryotes possess DNA-dependent RNA polymerases of a common type. EMBO J. 1983;2(8):1291–1294. doi: 10.1002/j.1460-2075.1983.tb01583.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Madon J., Zillig W. A form of the DNA-dependent RNA polymerase of Halobacterium halobium, containing an additional component, is able to transcribe native DNA. Eur J Biochem. 1983 Jun 15;133(2):471–474. doi: 10.1111/j.1432-1033.1983.tb07487.x. [DOI] [PubMed] [Google Scholar]
- Marsh J. L., Erfle M., Wykes E. J. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene. 1984 Dec;32(3):481–485. doi: 10.1016/0378-1119(84)90022-2. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Reiter W. D., Palm P., Zillig W. Analysis of transcription in the archaebacterium Sulfolobus indicates that archaebacterial promoters are homologous to eukaryotic pol II promoters. Nucleic Acids Res. 1988 Jan 11;16(1):1–19. doi: 10.1093/nar/16.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schallenberg J., Moes M., Truss M., Reiser W., Thomm M., Stetter K. O., Klein A. Cloning and physical mapping of RNA polymerase genes from Methanobacterium thermoautotrophicum and comparison of homologies and gene orders with those of RNA polymerase genes from other methanogenic archaebacteria. J Bacteriol. 1988 May;170(5):2247–2253. doi: 10.1128/jb.170.5.2247-2253.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shaw W. V. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 1975;43:737–755. doi: 10.1016/0076-6879(75)43141-x. [DOI] [PubMed] [Google Scholar]
- Thomm M., Sherf B. A., Reeve J. N. RNA polymerase-binding and transcription initiation sites upstream of the methyl reductase operon of Methanococcus vannielii. J Bacteriol. 1988 Apr;170(4):1958–1961. doi: 10.1128/jb.170.4.1958-1961.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomm M., Wich G. An archaebacterial promoter element for stable RNA genes with homology to the TATA box of higher eukaryotes. Nucleic Acids Res. 1988 Jan 11;16(1):151–163. doi: 10.1093/nar/16.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomm M., Wich G., Brown J. W., Frey G., Sherf B. A., Beckler G. S. An archaebacterial promoter sequence assigned by RNA polymerase binding experiments. Can J Microbiol. 1989 Jan;35(1):30–35. doi: 10.1139/m89-005. [DOI] [PubMed] [Google Scholar]
- Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
- Wich G., Hummel H., Jarsch M., Bär U., Böck A. Transcription signals for stable RNA genes in Methanococcus. Nucleic Acids Res. 1986 Mar 25;14(6):2459–2479. doi: 10.1093/nar/14.6.2459. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zillig W., Klenk H. P., Palm P., Pühler G., Gropp F., Garrett R. A., Leffers H. The phylogenetic relations of DNA-dependent RNA polymerases of archaebacteria, eukaryotes, and eubacteria. Can J Microbiol. 1989 Jan;35(1):73–80. doi: 10.1139/m89-011. [DOI] [PubMed] [Google Scholar]
- Zillig W., Palm P., Reiter W. D., Gropp F., Pühler G., Klenk H. P. Comparative evaluation of gene expression in archaebacteria. Eur J Biochem. 1988 May 2;173(3):473–482. doi: 10.1111/j.1432-1033.1988.tb14023.x. [DOI] [PubMed] [Google Scholar]




