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. 2012 Mar 15;7(3):e32661. doi: 10.1371/journal.pone.0032661

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(A) The secondary structures of the IVT RNAs were predicted using the program mfold (v3.2). (B) A549 cells were transfected with 3 µg of in vitro transcribed RNAs from the 5′ end of the cRNA/3′ end of the vRNA sequence of NS1 gene. 24 hr post-transfection, RNA was extracted to determine the levels of IFN-β by qRTPCR. The data are shown as folds over the mock control. Hatched bar, filled bar and empty bars represent untreated, CIP-treated and capped RNAs.