Figure 4. Smaller U/A rich IVT RNAs from the cRNA and vRNA UTRs are also triphosphate independent.

(A) The secondary structures of the IVT-RNAs used are presented. (B) A549 cells were transfected with U/A-rich cRNA and vRNA. 24 hours post-transfection, RNA was isolated to determine IFN-β mRNA levels by qRT-PCR. The data are shown as fold increases over levels in mock transfected cells. Hatched bar, filled bar and empty bars represent untreated, CIP-treated and capped RNAs. Error bars represent the standard deviation of triplicate qRT-PCR runs using RNAs from one of three representative experiments.