Figure 4. C. parvum Transcriptome 2–72 hr Relative Expression Cluster Analysis.

The DIANA algorithm was used to cluster each gene's relative expression using the absolute cosign distance metric. For the heatmap each gene is represented in one row and each column represents the percent expression at one time point. (A) The relative expression (%) was determined for each gene's median normalized value over the 7 time point, 72 hr time course (3302 genes). Nine clusters (1–9) are present with the dendrogram distance cutoff of 100 as indicated with the colored bar to the right of the heatmap. (B) For the 72 hr time course, each clusters' average expression (+/− SD) is graphed, with the number of genes found in each cluster within the parentheses.