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. 2012 Mar 15;7(3):e33737. doi: 10.1371/journal.pone.0033737

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Larvae of the indicated genotypes were dissected and vpda branch number was quantified. (A–C) Representative examples of vpda neurons labeled with mCD8-GFP and visualized by fluorescent microscopy. (A) rac1^J11rac2^Δmtl^Δ/GAL4^2–21UAS-mCD8-GFP, (B) UAS-Trio; GAL4^2–21UAS-mCD8-GFP, (C) UAS-Trio; rac1^J11rac2^Δmtl^Δ/GAL4^2–21UAS-mCD8-GFP. (D) quantification of vpda branch number. Vertical bars report number of branches for the indicated genotypes, lines indicate SEM. Number in each bar is the number of samples. Asterisks indicate p<0.05.