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. 2012 Mar 15;7(3):e33737. doi: 10.1371/journal.pone.0033737

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trio¹/trio^M89 mutant larvae were filleted and stained with the pan-neuronal antibody anti-HRP (green) and the indicated antibodies against class-specific markers (red). (A-A″) anti-Abrupt (Class I). Absence of Trio does not prevent expression of Abrupt in the Class I neurons ddaE or ddaF (arrows), nor does it induce expression of Abrupt in the Class IV neuron ddaC (arrowhead). (B-B″) anti-Knot (Class IV). Absence of Trio does not prevent expression of Knot in the Class IV neuron v' ada (arrow).