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. 2012 Mar 15;8(3):e1002564. doi: 10.1371/journal.pgen.1002564

Figure 2. Late induction of roX1 expression requires roX2 RNA.

Figure 2

In nuclei where dosage compensation was successfully turned on after late msl2 induction, extensive roX2 was observed painting the entire X chromosome. (A) However, only 1% of the chromosomes showed extensive roX1 painting. 34% and 59% of chromosomes showed roX1 expression confined to several Mbp around (B) or just at the endogenous roX1 locus (C), respectively. The remaining chromosomes (6%) had no roX1 expression despite the presence of roX2 (data not shown). roX1 and roX2 were detected by biotin (green, A–C) and digoxigenin (red, A′–C′) labeled antisense riboprobes, respectively. The merged figure is shown in A″–C″. White arrows denote the endogenous roX1 locus at band 3F.