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. 2012 Mar 15;8(3):e1002564. doi: 10.1371/journal.pgen.1002564

Figure 3. roX1 RNA is needed to sustain endogenous roX1 transcription in males.

Figure 3

X chromosomes from neighboring cells display a mosaic pattern in which the MSL complex either succeeded (arrowhead) or failed (arrow) to paint the X from roX2; msl2; H83M2 female (A) and male (B) salivary glands. (C) Endogenous roX1 and H83roX1Δ39 transcripts (Orange) and antisense riboprobe recognizing only full length roX1 (green). Whole mount roX1 FISH using the internal probe on salivary glands from (D) wild type male, (E) wild type female, (F) roX1+ roX2−/Y; msl2; H83M2 mosaic male, (G) roX1+roX2−/Y; msl2; H83M2 H83-roX1Δ39/+ male, (H) roX1− roX2 −/Y; msl2; H83M2 H83-roX1Δ39/+ male. The X chromosomes in G are fully painted in all cells with MSL complex relying upon roX1-Δ39 RNA (Figure S1D), but the truncated roX1 RNA is not recognized by the internal probe.