Wild type (WT) and Pcdh12−/− placentas and embryos were dissected at E12.5 (A, C, E) (n = 33 and 42, for WT and Pcdh12−/− individuals, respectively) and at E17.5 (B, D, F) (n = 46 and 33 for WT and Pcdh12−/− individuals, respectively). Placentas were weighed and their volume were calculated after measurement with a caliper, as described in Materials and Methods. Embryos were weighed after elimination of placenta and yolk sac. Histograms show (A, B) placenta weights, (C, D) placenta volumes, and (E, F) embryonic weights. Litter-mates from heterozygous X heterozygous matings were weighed from 2 to 8 weeks of postnatal age. Histograms in (G, H) represent postnatal weights of males (G) (n = 5 and 7, for WT and PCDH12-deficient mice, respectively) and females (H) (n = 7 and 6, for WT and PCDH12-deficient mice, respectively). Data represent the mean with SD. p-values are indicated when statistically significant differences were present.