Table 4.
Changes in expression of genes involved in cell-matrix adhesion and migration*
| Gene Symbol | Gene Product | Affymetrix ID | WT Signal Average | Pcdh12−/− Signal Average | Pcdh12−/− WT Signal Ratio | SAM Score | Q-Value |
|---|---|---|---|---|---|---|---|
| Matrix and matrix-related genes | |||||||
| Col1a1 | Procollagen, type I, alpha-1 | 1423669_at | 119.52 | 364.06 | 3.05 | 1.15 | 0.115 |
| Col1a2 | Procollagen, type I, alpha 2 | 1450857_a_at | 359.84 | 996.74 | 2.77 | 1.53 | 0.087 |
| Col4a2 | Procollagen, type IV, alpha 2 | 1424051_at | 904.66 | 2044.74 | 2.26 | 1.16 | 0.096 |
| Col5a1 | Procollagen, type V, alpha 1 | 1416740_at | 90.32 | 306.74 | 3.40 | 1.75 | 0.078 |
| Col5a2 | Procollagen, type V, alpha 2 | 1450625_at | 153.46 | 551.64 | 3.59 | 1.27 | 0.068 |
| Col6a3 | Procollagen type VI, alpha 3 | 1424131_at | 38.94 | 131.54 | 3.38 | 1.27 | 0.095 |
| Col15a1 | Procollagen, type XV, alpha 1 | 1426955_at | 67.24 | 150.2 | 2.23 | 1.35 | 0.058 |
| Plod2 | Procollagen lysine, 2-oxoglutarate 5-dioxygenase 2 | 1416687_at | 316.70 | 990.40 | 3.13 | 1.15 | 0.066 |
| Lox | Lysyl oxidase | 1416121_at | 337.24 | 893.10 | 2.65 | 0.78 | 0.112 |
| Lama1 | Laminin, alpha 1 | 1418153_at | 113.22 | 349.60 | 3.09 | 0.77 | 0.165 |
| Lama5 | Laminin, alpha 5 | 1427009_at | 312.54 | 706.98 | 2.26 | 1.83 | 0.078 |
| Lamb2 | Laminin, beta 2 | 1416513_at | 126.9 | 290.38 | 2.29 | 1.45 | 0.090 |
| Fbn1 | Fibrillin 1 | 1425896_a_at | 66.12 | 182.16 | 2.75 | 0.98 | 0.089 |
| Fbln2 | Fibulin 2 | 1423407_a_at | 131.9 | 324.54 | 2.46 | 1.04 | 0.083 |
| Fbln5 | Fibulin 5 | 1416164_at | 50.04 | 181.10 | 3.62 | 1.26 | 0.061 |
| Integrin genes | |||||||
| Itga3 | Integrin alpha 3 | 1421997_s_at | 49 | 140.4 | 2.87 | 1.45 | 0.082 |
| Itga6 | Integrin alpha 6 | 1422444_at | 205.18 | 292.2 | 1.42 | 1.17 | 0.124 |
| Itgav | Integrin alpha V | 1421198_at | 165.18 | 221.82 | 1.34 | 0.74 | 0.093 |
| Itgb1 | Integrin beta1D | 1452545_a_at | 696.92 | 916.86 | 1.32 | 1.01 | 0.098 |
| Itgb5 | Integrin beta 5 | 1417533_a_at | 755.58 | 1392.96 | 1.84 | 1.17 | 0.075 |
| Itgb7 | Integrin beta 7 | 1418741_at | 22.88 | 46.88 | 2.05 | 0.76 | 0.152 |
| Matrix protease and matrix protease inhibitor genes | |||||||
| Dcsn | Disintegrin metalloprotease (decysin) | 1419476_at | 8.90 | 1598.20 | 179.57 | 1.78 | 0.068 |
| Mmp9 | Gelatinase | 1416298_at | 15.0 | 35.44 | 2.41 | 0.88 | 0.141 |
| Mmp14 | Matrix metalloproteinase 14 | 1448383_at | 189.3 | 396.1 | 2.09 | 0.93 | 0.087 |
| Mmp23 | Matrix metalloproteinase 23 | 1417281_a_at | 14.44 | 43.98 | 3.05 | 0.61 | 0.238 |
| Adam9 | A disintegrin and metalloproteinase domain 9 | 1416094_at | 233.4 | 335.9 | 1.44 | 0.83 | 0.068 |
| Adam12 | A disintegrin and metalloproteinase domain 12 | 1421171_at | 58.06 | 100.96 | 1.74 | 0.50 | 0.189 |
| Adam17 | A disintegrin and metalloproteinase domain 17 | 1421858_at | 208.26 | 262 | 1.26 | 0.92 | 0.098 |
| Adam19 | A disintegrin and metalloprotease domain 19 | 1418403_at | 196.76 | 385.88 | 1.96 | 0.90 | 0.093 |
| Adamts2 | A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 2 | 1457058_at | 248.08 | 562.9 | 2.27 | 0.85 | 0.118 |
| Adamts9 | A disintegrin-like and metalloprotease with thrombospondin type 1 motif, 9 | 1431399_at | 28.76 | 47.94 | 1.67 | 0.77 | 0.143 |
| Timp2 | Tissue inhibitor of metalloproteinase 2 | 1450040_at | 39.44 | 74.6 | 1.89 | 0.81 | 0.103 |
*
Genes of these categories were selected as follows: lowest signal value above 15, SAM score above 0.50.