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NSP4 is secreted as complex with phospholipid. TLC/FID chromatograms of lipids extracted from purified NSP4, compared to standards (upper panel; AH = aliphatic hydrocarbon, FA = free fatty acid, Ch = cholesterol, PL = phospholipid). The major peak in the NSP4-derived sample corresponds to phospholipid. The minor peak marked *represents the ketone internal standard added to the NSP4 sample to permit quantification of the extracted lipid
