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PCR detection of EGFP gene and ORFV113 or ORFV116 in OV-IA82Δ113 and OV-IA82Δ116. Genomic DNA was isolated from OV-IA82 and OV-IA82Δ113 (A) or OV-IA82Δ116 (B). PCR was preformed to confirm the absence of ORFV113 (A) and ORFV116 (B) and the presence of the EGFP reporter gene sequences in OV-IA82Δ113 and OV-IA82Δ116 genomes. M: molecular marker (HyperLadder I, Bioline); Lane 1: OV-IA82; Lane 2 to 4: three different recombinants of OV-IA82Δ113 (A) and OV-IA82Δ116 (B).
