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Comparison of generating rORFVs using the pSPV-EGFP and pZIPPY-neo/gus vectors
| OV gene | Rounds of plaque assay | Time to obtain the puried rOVs (days) | ||
|---|---|---|---|---|
| pSPV-EGFP | pZIPPY-neo/gus | pSPV-EGFP | pZIPPY-neo/gus | |
| ORFV002 | -a | 16 | - | 80[23]b |
| ORFV012 | - | 20 | - | >100C |
| ORFV024 | - | 19 | - | 95[24] |
| ORFV113 | 4 | 20 | 12 | >100C |
| ORFV116 | 5 | 20 | 14 | >100C |
| ORFV120 | - | 17 | - | 85 |
| ORFV121 | - | 18 | - | 90[25] |
a Not tested; b Reference; c After 20 rounds of plaque assay, still contaminated with wild type virus.
