Table 2.
Oligonucleotide primers and restriction enzymes
| Primer | Sequence | Restriction enzyme* |
|---|---|---|
| GFPFw1 | 5'-AACTTAGAATTCGCCACCATGGTGAGCAAGGGCGA-3' | EcoR I |
| GFPRv1 | 5'-ATCAATGCGGCCGCTTACTTGTACAGCTCGTCCA-3' | Not I |
| GFPintrFw2 | 5'-GACGTAAACGGCCACAAGTT-3' | |
| GFPintrRv2 | 5'-ACTGGGTGCTCAGGTAGTGG-3' | |
| GFPseqFw3 | 5'-CGACCACTACCAGCAGAACA-3' | |
| GFPseqRv3 | 5'-AAGTCGTGCTGCTTCATGTG-3' | |
| 113LFFw1 | 5'-AGGCCTCTAAGCTTCAGGTTCCGGCTTCAGATGCGCGT-3' | Hind III |
| 113LFRv1 | 5'-ATTCGCGTCGACCACCAACACTTCCATTGTTGCGGC-3' | Sal I |
| 113RFFw2 | 5'-TCTTATGCGGCCGCGAGCCGCCGATGCAGATCGAGGTA-3' | Not I |
| 113RFRv2 | 5'-ATTCGCAGATCTTCGATCGCCAGTGCGCGGCGCATG-3' | Bgl II |
| 113intrFw3 | 5'-CGCCGTAATATGCTTAACCGGAGC-3' | |
| 113intrRv3 | 5'-CGGACCGTGTTGGTCGTTGGGTCT-3' | |
| 113seqFw4 | 5'-TTAGCTTCCTTGTTTTTATC-3' | |
| 113seqRv4 | 5'-GTCCTTCGGGTCAGAGTCC-3' | |
| 116LFFw1 | 5'-GCCTCTACTAGTAGGAAGTGGCCTCGCCGACCACGA-3' | Spe I |
| 116LFRv1 | 5'-ATTCGCGTCGACGTGGATGTCTCTAAGGTTCAATAC-3' | Sal I |
| 116RFFw2 | 5'-TCTTATGCGGCCGCCTACCACTGGTACCAGCACCTCCT-3' | Not I |
| 116RFRv2 | 5'-ATTCGCAGATCTGGCGCTACAGGCGTCCTGCAGGAA-3' | Bgl II |
| 116intrFw3 | 5'-GAACAACACGTCAACCGATG-3' | |
| 116intrRv3 | 5'-AGGTGTGGGTTGACTTCCAG-3' | |
| 116seqFw4 | 5'-GTCGAGCAGATGTTCATGGA-3' | |
| 116seqRv4 | 5'-ATGCTGCACTTCCTGGAGAT-3' | |
| 001intrFw1 | 5'-CTCGGTGACCTGCCTGAC-3' | |
| 001intrRv1 | 5'-CTCGCGCACGTCGTAGAT-3' |
* Restriction enzyme sites are underlined in prime sequences