Table 1.
Summary of in silico digestion results
| Restriction Enzyme | Fragments (200 -700 bp) | Distance between two adjacent reads | #Putative SNPsb | ||||||
|---|---|---|---|---|---|---|---|---|---|
| #Total selected fragments | #Total length of target regionsa | % Percent of coverage | #Length of repetitive on target regions | %percent of repetitive contents | Mean (Mb) | Median (Mb) | S.D.(Mb) | ||
| Sac I | 65,734 | 11,832,120 | 0.38% | 4,642,275 | 39.24% | 22,732 | 442 | 131.63 | 48,250 |
| Ava I | 69,204 | 12,456,720 | 0.40% | 5,388,814 | 43.26% | 21,582 | 368 | 140.26 | 59,204 |
| Hind III | 114,374 | 20,587,320 | 0.67% | 8,376,468 | 40.69% | 13,027 | 425 | 95.84 | 79,308 |
| Pvu II | 194,918 | 35,085,240 | 1.14% | 12,510,491 | 35.66% | 7,607 | 379 | 73.01 | 137,942 |
| Sfc I | 442,338 | 79,620,840 | 2.59% | 33,483,887 | 42.05% | 3,303 | 348 | 47.28 | 319,623 |
| Dra I | 1,131,481 | 203,666,580 | 6.61% | 74,549,862 | 36.60% | 1,237 | 235 | 29.41 | 774,892 |
| Tsp 45I | 1,479,019 | 266,223,420 | 8.64% | 104,133,241 | 39.12% | 926 | 224 | 25.64 | 1,074,049 |
| Bcc I | 2,419,310 | 435,475,800 | 14.14% | 216,911,945 | 49.81% | 531 | 170 | 19.95 | 1,750,903 |
| Mbo II | 3,308,660 | 595,558,800 | 19.33% | 251,315,078 | 42.20% | 365 | 148 | 17.04 | 2,298,087 |
The in silico digestion results of nine restriction enzymes using hg18 genome as reference were shown. a regions sequenced in the final corresponding library and calculated according to pair-end sequencing with average read length of 90 bp. b The number of putative SNPs are calculated based on dbSNP v129 data.