Table 6.
Primer sequences and conditions employed in RT-PCR
| Gene | Primer sequence | TA (°C) |
|---|---|---|
| p53 | 5'GGC CCA CTT CAC CGT ACT AA3' 5'GTG GTT TCA AGG CCA GAT GT3' |
60 |
| Mitogen activated protein kinase (MAPK1) |
5'CCA CCC ATA TCT GGA GCA GT3' 5'CAG TCC TCT GAG CCC TTG TC 3' |
60 |
| Interleukin-8 (IL-8) | 5'ATG ACT TCC AAG CTG GCC GTG GCT 3' 5'TCT CAG CCC TCT TCA AAA ACT TCT C 3' |
61 |
| Nuclear factor beta (NFκB) | 5' AGT TGA GGG GAC TTT CCC AGG 3' 5' GCC TGG GAA AGT CCC CTC AAC T 3' |
61 |
| 18s Ribosomal subunit | 5' GTA ACC CGT TGA ACC CCA TT-3' 5' CCA TCC AAT CGG TAG TAG CG 3' |
60 |