Table 3.

Sequencing coverage

Reference	Read Alignment	Control	T1	T2	T3
None	Total number of reads (million)	15.5	13.7	28.5	29.9
O.s. 6.11	Number of mapped reads (million)	13.4	11.8	24.6	26.4
O.s. 6.1	Complexity (Mb)	131.8	131.5	142.8	142.4
O.s. 6.1	Complexity, homozygous changes2 (Mb)	78.2	77.7	82.7	82.6
De novo3	Number of mapped reads (million)	13.3	11.7	24.5	26.3
De novo	Complexity (Mb)	102.4	102.1	106.9	106.7
De novo	Complexity, homozygous changes2 (Mb)	82.6	82.1	87.4	87.3

¹Reference complexity: 380 Mb. ²Complexity for homozygous scoring is calculated according to the following requirements: i) covered at least once in each of the four libraries, ii) covered at least twice in the putative mutant, iii) covered less than 200 times cumulatively in all libraries. ³Reference complexity: 117 Mb.