Figure 2.
Confocal images of nuclear accumulation of doxorubicin (20 μM) in the presence or absence of loperamide in MCF-7/MDR1 cells. (A)–(C) show the images from the cells after treatment for 3 hr, demonstrating increased nuclear accumulation of doxorubicin in the presence of loperamide. (A) Cells treated doxorubicin alone. (B) Cells treated with doxorubicin and 10 μM loperamide. (C) Cells treated with doxorubicin and 20 μM loperamide. (D)–(F) are the images from the cells after the removal of both doxorubicin and loperamide. (D) Cells pretreated with doxorubicin alone. (E) Cells pretreated with doxorubicin and 10 μM loperamide. (F) Cells pretreated with doxorubicin and 20 μM loperamide. The original magnification of all images was 400×, and the images were taken under the same microscope settings.