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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: Cell. 2012 Mar 2;148(5):1015–1028. doi: 10.1016/j.cell.2012.02.008

Figure 1. Slug is the major EMT-TF that is expressed in MaSCs.

Figure 1

(A) The mRNA levels of the EMT-TFs in MaSC-enriched basal (stem/basal) or luminal progenitor cells were compared to those of differentiated luminal cells (diff. luminal) by qRT-PCR in triplicate. GAPDH was used as a loading control.

(B) Slug protein expression in the mammary gland was analyzed by immunofluorescence on tissue sections. An anti-keratin 8 antibody was used to label the luminal cells.

(C) The expression of the Slug-YFP reporter in MaSC-enriched basal (basal/stem), luminal progenitor and differentiated luminal (diff. luminal) cells.

(D) The gland-reconstituting activities of Slug-YFP+ and Slug-YFP− MECs were measured by the limiting dilution analysis. Representative whole-mount images of carmine-stained fat pads (upper panel) and reconstitution efficiencies (lower panel) are shown. In the lower panel, each circle represents one transplanted fat pad, and the dark area of each circle represents the percentage of the fat pad that is occupied by reconstituted mammary ductal trees. P=1.6X10−5.

The data are represented as mean ± standard error of the mean (SEM). See also Figure S1.