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. Author manuscript; available in PMC: 2013 Mar 2.
Published in final edited form as: Cell. 2012 Mar 2;148(5):1015–1028. doi: 10.1016/j.cell.2012.02.008

Figure 2. The ectopic expression of Slug induces MaSC activity.

Figure 2

(A) The expression levels of EMT-associated proteins were determined by immunoblot. The primary MECs transduced with tetracycline-inducible Slug lentivirus were treated with the indicated concentration of doxycycline for 5 days.

(B) The organoid-forming efficiencies of the primary MECs transduced with the indicated vectors.

(C) The gland-reconstituting activity was measured using the competitive reconstitution assay. The left panel shows representative whole-mount fluorescence images of the mammary fat pads at the indicated time points post-injection. Right panel shows the ratios of GFP-expressing (either control vector or Slug) to dsRed-expressing cells, as measured by flow cytometry.

(D, E) The organoid-forming efficiencies of MaSC-enriched basal cells (D) or luminal progenitor cells (E) that were transduced with the indicated vectors.

The data are represented as mean ± SEM. See also Figure S2.

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