Table 3.
Plasmids
| Plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| pBP141 | pCR2.1 containing tadZ A155K Af/II fragment | This study |
| pBP149 | pCR2.1 containing tadZ A155K | This study |
| pBP154 | pJAK17 containing tadZ A155K | This study |
| pBP169 | pET30a containing tadZ | This study |
| pBP171 | pET30a containing tadA | This study |
| pBP174 | pCR2.1 containing tadZ S156T Af/II fragment | This study |
| pBP180 | pCR2.1 containing tadZ S156T | This study |
| pBP182 | pCR2.1 containing tadZ K150A Af/II fragment | This study |
| pBP184 | pCR2.1 containing tadZ K150R Af/II fragment | This study |
| pBP187 | pJAK17 containing tadZ S156T | This study |
| pBP193 | pCR2.1 containing tadZ K150R | This study |
| pBP195 | pCR2.1 containing tadZ K150A | This study |
| pBP196 | pCR2.1 containing tadZ S156A Af/II fragment | This study |
| pBP197 | pJAK17 containing tadZ K150R | This study |
| pBP200 | pJAK17 containing tadZ K150A | This study |
| pBP209 | pCR2.1 containing tadZ S156A | This study |
| pBP214 | pCR4 containing tadZ | This study |
| pBP215 | pCR4 containing tadZ A155K (BamHI-NcoI fragment) | This study |
| pBP216 | pCR4 containing tadZ S156A (BamHI-NcoI fragment) | This study |
| pBP219 | pJAK17 containing tadZ S156A | This study |
| pBP222 | pCR4 containing tadZ S156T (BamHI-NcoI fragment) | This study |
| pBP225 | pJAK17 containing tadZ-egfp | This study |
| pBP226 | pJAK17 containing tadZ S156A-egfp | This study |
| pBP227 | pEGFP containing tadZ | This study |
| pBP228 | pEGFP containing tadZ S156A | This study |
| pBP230 | pEGFP containing tadZ A155K | This study |
| pBP231 | pJAK17 containing tadZ A155K-egfp | This study |
| pBP236 | pEGFP containing tadZ S156T | This study |
| pBP237 | pCR4 containing tadZ K150A (BamHI-NcoI fragment) | This study |
| pBP238 | pCR4 containing tadZ K150R (BamHI-NcoI fragment) | This study |
| pBP244 | pJAK17 containing tadZ S156T-egfp | This study |
| pBP246 | pEGFP containing tadZ K150A | This study |
| pBP248 | pEGFP containing tadZ K150R | This study |
| pBP252 | pJAK17 containing tadZ K150A-egfp | This study |
| pBP254 | pJAK17 containing tadZ K150R-egfp | This study |
| pBP287 | pCR2.1 containing tadZ (BamHI-Sa/I fragment) | This study |
| pBP294 | pJH391 containing tadZ | This study |
| pBP296 | pCR2.1 containing tadZ K150R (BamHI-Sa/I fragment) | This study |
| pBP297 | pCR2.1 containing tadZ K150A (BamHI-Sa/I fragment) | This study |
| pBP298 | pCR2.1 containing tadZ S156T (BamHI-Sa/I fragment) | This study |
| pBP299 | pCR2.1 containing tadZ S156A (BamHI-Sa/I fragment) | This study |
| pBP300 | pCR2.1 containing tadZ A155K (BamHI-Sa/I fragment) | This study |
| pBP301 | pCR2.1 containing λcIDB-tadZ | This study |
| pBP303 | pJH391 containing tadZ K150A | This study |
| pBP305 | pJH391 containing tadZ S156T | This study |
| pBP307 | pCR2.1 containing tadA | This study |
| pBP308 | pEGFP containing tadZΔA1 | This study |
| pBP310 | pJAK16 containing λcIDB-tadZ | This study |
| pBP312 | pEGFP containing tadA | This study |
| pBP318 | pJH391 containing tadZ S156A | This study |
| pBP319 | pJH391 containing tadZ A155K | This study |
| pBP320 | pEGFP containing tadZΔA2 | This study |
| pBP321 | pJAK17 containing tadA-Δegfp | This study |
| pBP323 | pJH391 containing tadZ K150R | This study |
| pBP325 | pJAK17 containing tadZΔA1-egfp | This study |
| pBP327 | pJAK17 containing tadZΔA2-egfp | This study |
| pBP339 | pCR2.1 containing tadZΔA1 | This study |
| pBP340 | pCR2.1 containing tadZΔA2 | This study |
| pBP346 | pJAK17 containing tadZΔA1 | This study |
| pBP352 | pJAK17 containing tadZΔA2 | This study |
| pCR2.1 | E. coli cloning vector; Kmr Apr | Invitrogen |
| pCR4 | E. coli cloning vector; Kmr Apr | Invitrogen |
| pEGFP | E. coli EGFP cloning vector; Apr | Clontech |
| pEK2 | pJAK16 containing tadA | (Bhattacharjee et al.,2001) |
| pET30a | E. coli protein expression vector; Kmr | Novagen |
| pGS-GFP | IncQ broad-host-range expression vector pMMB207 encoding GFP; Cmr | (Hilbietal., 2001) |
| pJAK16 | IncQ broad-host-range expression vector; tacp+, Cmr | J. Kornacki, unpublished |
| pJAK17 | IncQ broad-host-range expression vector; tacp+, Cmr | J. Kornacki, unpublished |
| pPP26 | pCR2.1 containing tadZ | This study |
| pPP29 | pJAK16 containing tadZ | (Planet et al., 2003) |
| pZ150 | Vector control for pJH391 and derivatives; Apr | (Hu et al., 1990) |
a
Apr, ampicillin resistance; Kmr, Kanamycin resistance; Cmr, chloramphenicol resistance.