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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Kidney Int. 2012 Jan 18;81(7):662–673. doi: 10.1038/ki.2011.443

Figure 4. Effects of GSK3β inhibition on the induction of the MPT by DCLF in mouse kidneys.

Figure 4

A: Mitochondrial swelling was spectrophotometrically monitored at 540 nm following induction with 20 M CaCl2 for 20 min. B: Change in absorbance at OD540 nm after 20 min. C: Correlation between decreased absorbance at 540 nm and the renal tubular injury score. D: Correlation between decreased absorbance at 540 nm and the number of apoptotic cells in renal tissues. Group Ctrl, control (vehicle treated); Group T, TDZD-8 (5 mg/kg); Group D, DCLF (200 mg/Kg); Group T-L+D, TDZD-8 (1 mg/Kg)+DCLF (200 mg/Kg); Group T+D, TDZD-8 (5 mg/Kg)+DCLF (200 mg/Kg). *P<0.05 versus Group Ctrl; #P<0.05 versus Group D. The decrease in absorbance at 540 nm was negatively correlated with the number of apoptotic cells and the renal tubular injury scores in mouse kidneys. Mitochondria that were isolated from kidneys treated with DCLF displayed an increased rate of mitochondrial swelling and a greater decrease in absorbance at 540 nm versus the controls, while TDZD-8 treatment ameliorated this process in a dose-dependent fashion.