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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Invest Dermatol. 2011 Dec 15;132(4):1103–1110. doi: 10.1038/jid.2011.412

Figure 2. AKR1C3 protein and mRNA are upregulated in response to calcium-induced PHK differentiation in a time and dose dependent manner.

Figure 2

(a) Western blot analysis demonstrating the regulation of AKR1C3 by PHK cultured under 1.8 mM or 0.03mM calcium conditions and harvested at intervals or (b) by cells treated with the indicated dose of calcium and harvested 48 hours post treatment (representative results of 3 separate experiments is shown). (c, d) RT-PCR analysis demonstrating the regulation of AKR1C3 mRNA by cells treated under the same conditions described in a or b, respectively. Data is expressed as means ± SEM fold change over the baseline (n=3, *P<0.015).