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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Invest Dermatol. 2012 Jan 26;132(4):1188–1195. doi: 10.1038/jid.2011.447

Figure 4. Defects in E-cadherin localization Are Normalized by Inhibiting SGPL1.

Figure 4

A-D) Keratinocytes cultured in 1.2 mM Ca2+ for 48 hours were stained with an E-cadherin antibody that recognizes an extracellular epitope. E-H) Keratinocytes cultured in 1.2 mM Ca2+ for 48 hours stained with an E-cadherin antibody that recognizes an intracellular epitope. (A and E) Control keratinocytes. (B and F) After treatment with 10 nM TG, extracellular E-cadherin staining (B) is discontinuous, with filamentous strands (arrows). (F) Perinuclear accumulation of E-cadherin is seen (arrows). (C and G) Scrambled siRNA does not rescue intracellular or extracellular E-cadherin localization (arrows). (D and H) SGPL1 inhibition with siRNA restores continuous, fine extracellular E-cadherin localization (D) and reverses the E-cadherin intracellular retention (H) seen after SERCA2 inhibition with TG.