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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Invest Dermatol. 2012 Jan 26;132(4):1188–1195. doi: 10.1038/jid.2011.447

Figure 6. Defects in Desmoplakin (DP) localization, Caused by SERCA2 inactivation with TG, Are Normalized by Inhibiting SGPL1.

Figure 6

Desmoplakin staining of human keratinocytes treated with 10nM TG, 3hours (A-F) and 48 hours (G-L) after raising extracellular Ca2+. In control (A), SGPL1 (B) and scrambled siRNA-treated cells (C), DP accumulation at cell-to-cell borders is evident within 3 hours after raising extracellular Ca2+. (G-I) 48 hours after raising extracellular Ca2+, DP localizes exclusively at the cell to cell borders in control (G) , SGPL1 siRNA (H) and scrambled siRNA-treated (I) cells. Treatment with 10 nM TG reduces DP staining at the cell borders three hours after raising extracellular Ca2+ (D) and intracellular retention of DP is evident at 48 hours (J); SGPL1 inhibition with siRNA improves DP localization at 3 hrs (E), but complete normalization of DP localization is seen only at 48 hours (K). Scrambled siRNA had no effect (F and L).