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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Invest Dermatol. 2012 Jan 26;132(4):1188–1195. doi: 10.1038/jid.2011.447

Figure 7. Defects in ER Ca2+ Sequestration are Induced by ER SERCA2 Inhibition, and are Normalized by Inhibiting SGPL1.

Figure 7

Primary normal human keratinocytes were transfected with the ER-targeted Ca2+ sensor D1ER (see Methods). Cells also were transfected with SGPL1 siRNA or scrambled siRNA as a control. 8 hours after siRNA transfection, cells were treated with 10 nM thapsigargin for two hours. The cells then were washed, and incubated in high calcium media for an additional 24 hours. A) average FRET ratio; B) average ER Ca2+ concentration; C) Relative Ca2+ changes between control (black bars) and TG treated cells (grey bars) in untreated, scrambled siRNA, and SGPL1 siRNA treated samples. Data are presented as the mean +/− s.e.m. N=15–29 cells from two independent sets of experiments in each group. Significance was calculated using a one-way ANOVA. Distributions with p<0.05 were assumed as statistically different.