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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Adv Drug Deliv Rev. 2011 Sep 29;64(6):571–588. doi: 10.1016/j.addr.2011.09.010

Figure 5.

Figure 5

Transepithelial permeability of 14C-mannitol and tight junction modulation in presence of PAMAM dendrimers; (a). The permeability of 14C-mannitol (3.3 μM) in the presence of fluorescently labeled and unlabeled PAMAM dendrimers of donor concentration 1.0 mM across Caco-2 cell monolayers at incubation times of (□) 60 min and (■) 120 min. Permeability values are not reported (**) for dendrimers that cause toxicity. Results are reported as mean +/− standard error of the mean (n = 9). (*) Denotes a significant increase in permeability compared to control (P < 0.001). (b). Transepithelial electrical resistance of Caco-2 cells in the presence of fluorescently labeled PAMAM dendrimers as a function of time: (◆) HBSS transport medium, (■) G2.0-NH2, (▲) G2.0-OH, (×) G1.5-COOH, (+) G2.5-COOH, (●) G3.5-COOH, (◇) G4NH2-FITC (1:8). Results are reported as mean +/− standard error of the mean (n = 9). (c). Staining of the tight junction protein occludin. (A) Caco-2 cells with no polymer treatment. Caco-2 cells incubated for 120 min with 1.0 mM: (B) G2.0-NH2; (C) G2.0-OH, (D) G1.5-COOH; (E) G2.5-COOH; (F) G3.5-COOH. Main panels illustrate the xy plane; horizontal bars illustrate the xz plane; vertical bars illustrate the yz plane. Scale bars equal 100.00 μm. With permission from Ref [15].